magnetic negative selection (Miltenyi Biotec)
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magnetic negative selection
Magnetic Negative Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+magnetic+selection/Naive+CD4%2B+T+Cell+Isolation+Kit+II%2C+human/pmc13284711-33-11-21
Average 96 stars, based on 207 article reviews
Magnetic Negative Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+magnetic+selection/Naive+CD4%2B+T+Cell+Isolation+Kit+II%2C+human/pmc13284711-33-11-21
Average 96 stars, based on 207 article reviews
magnetic negative selection - by Bioz Stars,
2026-09
96/100 stars
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Selection:Article Title: A CAR-T Cell-Based Strategy for Eliminating Pathogenic Microglia in ALS Article Snippet: Human T cells were isolated from PBMCs obtained from healthy donor leukopaks (200-0092, Stemcell Technologies). .. Article Title: Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells Article Snippet: .. Pan T cells were isolated from PBMC of two healthy donors using Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis Article Snippet: Primary NK cells were isolated from mouse spleens using negative magnetic separation (STEMCELL Technologies, cat. 19855) and maintained in RPMI with 10% FBS, in 5% CO 2 at 37°C. .. Primary CD4 + T cells were isolated from mouse spleens using Article Title: Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells Article Snippet: .. Pan T cells were isolated from PBMC of two healthy donors using Article Title: Epigenetic, transcriptional, and functional characterization of myeloid cells in familial Mediterranean fever Article Snippet: The PBMCs were washed three times with cold PBS and counted by a Sysmex XN-450 apparatus. .. Immediately afterwards, monocytes were isolated using Article Title: Loss of function of XBP1 splicing activity of IRE1α favors B cell tolerance breakdown. Article Snippet: Anti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma.. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known.. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. Article Title: Severe combined immunodeficiency in stimulator of interferon genes (STING) V154M/wild-type mice. Article Snippet: 571 572 Cell preparation and culture 573 M AN US CR IP T AC CE PT ED ACCEPTED MANUSCRIPT 20 In vitro B- and T-cell stimulations were performed in complete RPMI-1640 medium 574 containing L-glutamine (Lonza) supplemented with 10% FCS (Dutscher), 50 mM β-575 Mercaptoethanol (Gibco) 1% Penicillin/Streptomycin (Gibco), 10 mM HEPES (Lonza), and 1 576 mM Sodium Pyruvate (Lonza). .. Splenic naïve (CD43 negative) B cells were purified by 577 Article Title: CXCL12/CXCR4 Axis Drives the Chemotaxis and Differentiation of B Cells in Bullous Pemphigoid. Article Snippet: Bullous pemphigoid (BP) is an autoimmune bullous skin disease characterized by autoantibodies against the hemidesmosomal proteins in the skin and mucous membranes.. The efficiency of B-cell‒targeting biologics in BP indicates the important role of B cells in its pathogenesis.. However, abnormal B-cell migration and differentiation in BP require further elucidation. Isolation:Article Title: Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells Article Snippet: .. Pan T cells were isolated from PBMC of two healthy donors using Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis Article Snippet: Primary NK cells were isolated from mouse spleens using negative magnetic separation (STEMCELL Technologies, cat. 19855) and maintained in RPMI with 10% FBS, in 5% CO 2 at 37°C. .. Primary CD4 + T cells were isolated from mouse spleens using Article Title: Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells Article Snippet: .. Pan T cells were isolated from PBMC of two healthy donors using Article Title: Epigenetic, transcriptional, and functional characterization of myeloid cells in familial Mediterranean fever Article Snippet: The PBMCs were washed three times with cold PBS and counted by a Sysmex XN-450 apparatus. .. Immediately afterwards, monocytes were isolated using Article Title: CXCL12/CXCR4 Axis Drives the Chemotaxis and Differentiation of B Cells in Bullous Pemphigoid. Article Snippet: Bullous pemphigoid (BP) is an autoimmune bullous skin disease characterized by autoantibodies against the hemidesmosomal proteins in the skin and mucous membranes.. The efficiency of B-cell‒targeting biologics in BP indicates the important role of B cells in its pathogenesis.. However, abnormal B-cell migration and differentiation in BP require further elucidation. Cell Culture:Article Title: PD-1 Bispecific Killer Engager (PD-1 BiKE) effectively depletes effector T lymphocytes in experimental autoimmune encephalomyelitis Article Snippet: Primary NK cells were isolated from mouse spleens using negative magnetic separation (STEMCELL Technologies, cat. 19855) and maintained in RPMI with 10% FBS, in 5% CO 2 at 37°C. .. Primary CD4 + T cells were isolated from mouse spleens using Magnetic Cell Separation:Article Title: Epigenetic, transcriptional, and functional characterization of myeloid cells in familial Mediterranean fever Article Snippet: The PBMCs were washed three times with cold PBS and counted by a Sysmex XN-450 apparatus. .. Immediately afterwards, monocytes were isolated using Article Title: Loss of function of XBP1 splicing activity of IRE1α favors B cell tolerance breakdown. Article Snippet: Anti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma.. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known.. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. Article Title: Severe combined immunodeficiency in stimulator of interferon genes (STING) V154M/wild-type mice. Article Snippet: 571 572 Cell preparation and culture 573 M AN US CR IP T AC CE PT ED ACCEPTED MANUSCRIPT 20 In vitro B- and T-cell stimulations were performed in complete RPMI-1640 medium 574 containing L-glutamine (Lonza) supplemented with 10% FCS (Dutscher), 50 mM β-575 Mercaptoethanol (Gibco) 1% Penicillin/Streptomycin (Gibco), 10 mM HEPES (Lonza), and 1 576 mM Sodium Pyruvate (Lonza). .. Splenic naïve (CD43 negative) B cells were purified by 577 Purification:Article Title: Loss of function of XBP1 splicing activity of IRE1α favors B cell tolerance breakdown. Article Snippet: Anti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma.. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known.. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. Article Title: Severe combined immunodeficiency in stimulator of interferon genes (STING) V154M/wild-type mice. Article Snippet: 571 572 Cell preparation and culture 573 M AN US CR IP T AC CE PT ED ACCEPTED MANUSCRIPT 20 In vitro B- and T-cell stimulations were performed in complete RPMI-1640 medium 574 containing L-glutamine (Lonza) supplemented with 10% FCS (Dutscher), 50 mM β-575 Mercaptoethanol (Gibco) 1% Penicillin/Streptomycin (Gibco), 10 mM HEPES (Lonza), and 1 576 mM Sodium Pyruvate (Lonza). .. Splenic naïve (CD43 negative) B cells were purified by 577 |